Improved sensitivity for meticillin resistance detection in coagulase-negative staphylococci by moxalactam antibiotic discs or a cefoxitin investigation zone

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Evaluation of oxacillin and cefoxitin disks for detection of resistance in coagulase negative staphylococci.

Coagulase-negative Staphylococcus spp. was considered nonpathogenic until the emergence of multiresistance and the demonstration of their participation as infectious agents. In Brazil, oxacillin resistance may be present in over 80% of isolates, and the Clinical and Laboratory Standards Institute standardized a disk-diffusion method to predict this resistance in Staphylococcus. The aim of this ...

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Methods for improved detection of oxacillin resistance in coagulase-negative staphylococci: results of a multicenter study.

A multilaboratory study was undertaken to determine the accuracy of the current National Committee for Clinical Laboratory Standards (NCCLS) oxacillin breakpoints for broth microdilution and disk diffusion testing of coagulase-negative staphylococci (CoNS) by using a PCR assay for mecA as the reference method. Fifty well-characterized strains of CoNS were tested for oxacillin susceptibility by ...

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Antibiotic resistance of coagulase-negative staphylococci and micrococci.

Five hundred and seventy strains of Gram-positive, catalase-positive, coagulasenegative cocci from various sources were classified on the basis of their ability to grow anaerobically and to ferment mannitol. Five groups were distinguished. The frequency of strains classified in the five groups varied with the source. Only 26% of the strains were sensitive to all the nine antibiotics used in the...

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Is the cefoxitin disk test reliable enough to detect oxacillin resistance in coagulase-negative staphylococci?

Coagulase-negative staphylococci (CoNS) represent an important etiology of nosocomial bloodstream infections (2, 4, 6). The introduction of empirical treatment has been a crucial step in reducing morbidity and mortality caused by such infections and in controlling the spread of resistant strains (1, 6). It has been advocated that the cefoxitin disk would be more sensitive than the 1g oxacillin ...

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Antibiotic susceptibility pattern and biofilm formation in coagulase negative staphylococci.

Introduction Coagulase negative staphylococci (CoNS) are commensals of non-sterile sites in humans and become pathogenic mostly when the host is immunocompromised by prior diseases or invasive surgical or related procedures [1]. Slime or biofilm production by CoNS has been identified as an important factor in the pathogenesis of infections as bacteria organized in biofilms are protected from th...

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ژورنال

عنوان ژورنال: Journal of Medical Microbiology

سال: 2016

ISSN: 0022-2615,1473-5644

DOI: 10.1099/jmm.0.000243